Device

Part:BBa_M39022

Designed by: Edgar Aranda-Michel   Group: MIT BE 20.20-S12   (2012-05-13)


Kill Switch

In the absence of tetR, this device will make granulysin and DNase1 which will kill the cell.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 63
    Illegal XbaI site found at 623
    Illegal PstI site found at 696
    Illegal PstI site found at 1229
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 696
    Illegal PstI site found at 1229
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 63
    Illegal XbaI site found at 623
    Illegal PstI site found at 696
    Illegal PstI site found at 1229
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 63
    Illegal XbaI site found at 623
    Illegal PstI site found at 696
    Illegal PstI site found at 1229
    Illegal AgeI site found at 376
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 750
    Illegal BsaI.rc site found at 1241
    Illegal SapI site found at 1401


[edit]
Categories
Parameters
None